Insights into the polyphenols extraction from Actinidia arguta fruit (kiwiberry): A source of pro-healthy compounds

Actinidia arguta fruit (also known as kiwiberry) has a remarkable phytochemical profile, being reported promising preventive agent/treatment for chronic diseases and desirable source of bioactive molecules pharmaceutical cosmetic industries. Despite these indications, investigating the full potential A. remains an unmet need health applications. This work aims to determine optimal extraction conditions antioxidant/antiradical compounds from (commonly kiwiberry), using Response Surface Methodology (RSM). The effects probe amplitude (30%-70%), sonication time (5-30 min) water:ethanol solvent ratio (0%-100%) were assessed. achieved 50% water, during 17.5 min 50%. A total 22 compounds, including 6 flavonoids 4 phenolic acids, identified in extract through HPLC-ESI-QTOF-MS, which exhibited outstanding antioxidant antiradical activities (TPC = 18.705 mg GAE/g dw; FRAP 186.876 µmol FSE/g ABTS 16.334 AAE/g O2·− IC50 829.384 µg/mL; HOCl 16.895 ROO· 0.18 µg TE/mg dw). Moreover, it displayed antimicrobial activity against Staphylococcus aureus (MIC 32 mg/mL) Pseudomonas gingivalis 64 reduced growth rate Escherichia coli. In-vitro assays demonstrated that successfully decreased viability two human carcinoma cell lines, namely TR146 HSC-3, at 500 µg/mL above µg/mL, respectively. present study showed is rich with be used pro-healthy purposes.